FIG 7.

Enhancement of stability of RpoS mRNA by cFP. (A) The relative mRNA level of rpoS after rifampin treatment was measured by qRT-PCR. Wild-type V. vulnificus MO6-24/O and the ΔvhuAB deletion mutant were treated with rifampin when growth in AB broth reached an A₆₀₀ of 0.2. At 0, 5, 10, 20, and 40 min after the treatment, RNA was isolated from cells for qRT-PCR analysis. Half-lives were calculated using GraphPad Prism 5. The data are average values from three independent samples, and the error bars denote standard deviations. (B) Translation level of rpoS as measured by β-galactosidase activity and Western hybridization using antiserum against RpoS of V. vulnificus wild type (pBBR1-MCS2), ΔvhuAB(pBBR1-MCS2), and ΔvhuAB(pBBR12-vhuAB) harboring pRZtl-rpoS cultured in AB broth. Measurements of β-galactosidase activities and Western hybridization were performed as described in Materials and Methods. The error bars denote standard deviations of the results of three independent experiments. MU, Miller units. **, P < 0.005; *, P < 0.05; NS, not significant).