FIG 2.

metJ deletion results in disruption of methionine metabolism. (A) MetJ regulates the generation of methionine and SAM in S. Typhimurium by transcriptionally repressing the methionine regulon. Black arrows represent enzymes transcriptionally repressed by MetJ. (B to E) Deletion of metJ leads to increased methionine (B), MTA (C), SAM (D), and phenylalanine (E) levels, as measured by mass spectrometry (n = 5 biological replicates). (F, G) metJ deletion did not affect spermidine concentrations (F) but did result in decreased amounts of spermine (G). P values, indicated at the top, were generated through a one-way analysis of variance with Tukey's multiple-comparison test. (H) metJ deletion did not affect bacterial growth in LB (n = 3 biological replicates grown in LB plus 0.5% DMSO). All error bars represent the standard error of the mean.