FIG 2.

Parasite antigen-specific immune responses elicited by the transgenic Eimeria lines. (A) Et-EmPro and Et-EmTgPro stimulate higher Eimeria parasite antigen-specific cellular immune responses than the wild type and Et-TgPro. The numbers of IFN-γ secretion lymphocytes (spots) in PBMCs from naive birds (Ctrl) and birds immunized with wild-type E. tenella (WT) or transgenic Eimeria parasites are shown. (B) Mean numbers of E. tenella antigen-specific IFN-γ secretion lymphocytes in PBMCs in birds immunized with transgenic Eimeria parasites or the wild type (n = 6). (C) The E. tenella antigen-specific antibody titer increases after primary self-boosting immunization with both transgenic Eimeria parasites and the wild type. Each value represents the mean for six birds. Each panel represents three independent experiments. **, P ≤ 0.01.