Protection of the birds immunized with transgenic Eimeria parasites after wild-type E. tenella challenge. Oocyst output (A and D), body weight gain (B and E), and cecum lesion scores (C and F) after challenge with E. tenella in the chickens immunized with or without transgenic Eimeria parasites or the wild type at 14 dpi (A to C) or 28 dpi (D to F) are shown. The birds were challenged with wild-type E. tenella at 10,000 oocysts per bird at 14 dpi and with 100,000 oocysts per bird at 28 dpi. Each value represents the mean for five birds. Each panel represents three independent experiments. *, P ≤ 0.05.