Table 2.
Species | Targets | Primer name | Primer sequence | References |
---|---|---|---|---|
Termitomyces sp. | mtSSU-rDNA | ssufw105 | TCGCGTTAGCATCGTTACTAGT | Aanen et al. 2002 |
ssurev475 | GCCAGAGACGCGAACGTTAGTCG | |||
EF1a | EF595F | CGTGACTTCATCAAGAACATG | Nobre et al. 2011b | |
EF1160R | CCGATCTTGTAGACGTCCTG | |||
RPB2 | RPB2-tF | GCG(GA)CGGAAAGACGACATCAG | ||
RPB2-tR | TTGTGATCAGGGAATGGGAT | |||
RPB2 | bRPB2-6F | TGGGGYATGGTNTGYCCYGC | De Fine Licht et al. 2006 | |
bRPB2-7 | CCCATRGCYTGYTTMCCCATDGC | |||
RPB1 | RPB1-AF | GARTGYCCDGGDCAYTTYGG | ||
Frpb1-CR | CCNGCDATNTCRTTRTCCATRTA | |||
EF1α | EF634F* | AGGCTGACTGCGCTATCCTTAT | ||
EF1127R* | GGTTCGATGGCATCGATGGCAT | |||
A. areolatum | nuc-IGS-rDNA | P-1 | TTGCAGACGACTTGAATGG | Nielsen et al. 2009 |
5S-2B | CACCGCATCCCGTCTGATCTGCG | |||
RPB2 | bRPB2-6F | TGGGGYATGGTNTGYCCYGC | Bergeron et al. 2011 | |
bRPB2-7.1R | CCCATRGCYTGYTTMCCCATDGC | |||
TEF1 | tef1f | TCMAHGARATYATYAAGGAGAC | ||
tef1rc | DGGGTCGTTYTTSGAGTCA | |||
LAC-like | laccasef | CACTGGCACGGNTTCTTCCA | ||
laccaserc | GTGACTATGATACCAGAANGT | |||
MAP | MIPAro1F* | GTCCTTTCACTCTTCGGTAC | Van Der Nest et al. 2008 | |
MIPAro1R* | CAAATAACTGGCGCCATACC | |||
PAB1 | RAB1-470F* | TCTTGGGCTGACTTTTCC | ||
RAB1-1800R* | GGCAGGTAGATCGAGGTTGA | |||
mat-B | br1-F | TGGCATMTNCARGCNTGGAAYTC | ||
br1-1R | GCGAGNRNCATNAGNCGNAKGTA | |||
Septobasidiaceae fungi | TEF-1 | SEF1a1fI | CTYGGIAAGGGITCNTTCAAG | Henk and Vilgalys 2007 |
SEF1a1r2 | CATICCGGCCTTGATNGTNCC | |||
O. sinensis | rDNA | OsT-F* | GTCAAGAAGCAAGCAAAGGAATC | Zhong et al. 2014 |
OsT-R* | TCAACTGGAGGGTGTGGTGG | |||
MAT1-2-1 | Mat1-2F* | TGGAATGCGACTGACTACGA | Zhang et al. 2011** | |
Mat1-2R* | CCAGGAGAGCTTGCTTGACT | |||
MAT1-1-1 | MATF2 | AAACGCCCTCTCAAYGCNTTYATG | Bushley et al. 2013** | |
MATR3 | CCACTTGCTTCTGAANGGRTCYTTRTTCCA | |||
DNA lyase | DNAF3 | TTGATGGGATCCGAYCAYTGYCC | ||
DNAR2 | GTGACCAGGCTGATRCANGGYTC | |||
MAT1-1-3 | M3F1 | CAGCAGCCGGTGAAGGTNTWYCAYGA | ||
M3R1 | CTTCGTTGTGTACTTGTANYCNGGRTA | |||
β-Tubulin | T1 | AACATGCGTGAGATTGTAAGT | Quan et al. 2014 | |
T22 | TCTGGATGTTGTTGGGAATCC | |||
SSU rRNA | NS1 | GTAGTCATATGCTTGTCTC | Zhong et al. 2010** | |
NS4 | CTTCCGTCAATTCCTTTAAG | |||
C. militaris | MAT1-1-1 | MAT1-F1 | CGRGCWAARCGRCCATTKAAYGC | Yokoyama et al. 2006** |
MAT1- R1 | TTKCCCATCTCRTCRCGGAYRAARGA | |||
MAT1- 2-1 | MAT2 -F1 | GCRTATA TTCT RT ACCGC AG | ||
MAT2-R1 | CGAGGTT GATAYTGAT AYT G | |||
T. indicum complex | β-Tubulin | Bt2a | GGTAACCAAATCGGTGCTGCTTTC | Wang et al. 2006a |
Bt2b | ACCCTCAGTGTAGTGACCCTTGGC | |||
T. melanosporum | MAT1-1-1 | P19* | CAATCTCACTCGTGATGTCTGGGTC | Rubini et al. 2011b** |
P20* | TCTCGGGCTGGAGGTGCGGGTCGAGT | |||
MAT1-2-1 | P1* | CAGGTCCGTCATCTCCTTCCAGCAG | ||
P2* | CCACATGCGACCGAGAATCTTGGCTA | |||
Tuber truffles | EF1α | Tuber_f* | AGCGTGAGCGTGGTATCAC | Bonito et al. 2013** |
Tuber_r* | GAGACGTTCTTG ACGTTGAAG | |||
RPB2 | Tuber_f* | YAAYCTGACYTTRGCYGTYAA | ||
Tuber_r* | CRGTTTCCTGYTCAATCTCA | |||
PKC gene | pkc1f | CCCAAAGGTGGTCACGAAGTGTA | Wang et al. 2006b** | |
pkc1r | TGATGAACTCCTTCTTCAGAACC | |||
β-Tubulin | Bt1a | TTCCCCCGTCTCCACTTCTTCATG | ||
Bt1b | GACGAGATCGTTCATGTTGAACTC |
Note: *The special primers; **there are other primers for the target in the reference. The references of black body present available universal or special primers of ITS.