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. 2015 May 18;6(2):94–109. doi: 10.1080/21501203.2015.1043968

Table 2.

Common molecular markers and for representative macrofungi.

Species Targets Primer name Primer sequence References
Termitomyces sp. mtSSU-rDNA ssufw105 TCGCGTTAGCATCGTTACTAGT Aanen et al. 2002
ssurev475 GCCAGAGACGCGAACGTTAGTCG
EF1a EF595F CGTGACTTCATCAAGAACATG Nobre et al. 2011b
EF1160R CCGATCTTGTAGACGTCCTG
RPB2 RPB2-tF GCG(GA)CGGAAAGACGACATCAG
RPB2-tR TTGTGATCAGGGAATGGGAT
RPB2 bRPB2-6F TGGGGYATGGTNTGYCCYGC De Fine Licht et al. 2006
bRPB2-7 CCCATRGCYTGYTTMCCCATDGC
RPB1 RPB1-AF GARTGYCCDGGDCAYTTYGG
Frpb1-CR CCNGCDATNTCRTTRTCCATRTA
EF1α EF634F* AGGCTGACTGCGCTATCCTTAT
EF1127R* GGTTCGATGGCATCGATGGCAT
A. areolatum nuc-IGS-rDNA P-1 TTGCAGACGACTTGAATGG Nielsen et al. 2009
5S-2B CACCGCATCCCGTCTGATCTGCG
RPB2 bRPB2-6F TGGGGYATGGTNTGYCCYGC Bergeron et al. 2011
bRPB2-7.1R CCCATRGCYTGYTTMCCCATDGC
TEF1 tef1f TCMAHGARATYATYAAGGAGAC
tef1rc DGGGTCGTTYTTSGAGTCA
LAC-like laccasef CACTGGCACGGNTTCTTCCA
laccaserc GTGACTATGATACCAGAANGT
MAP MIPAro1F* GTCCTTTCACTCTTCGGTAC Van Der Nest et al. 2008
MIPAro1R* CAAATAACTGGCGCCATACC
PAB1 RAB1-470F* TCTTGGGCTGACTTTTCC
RAB1-1800R* GGCAGGTAGATCGAGGTTGA
mat-B br1-F TGGCATMTNCARGCNTGGAAYTC
br1-1R GCGAGNRNCATNAGNCGNAKGTA
Septobasidiaceae fungi TEF-1 SEF1a1fI CTYGGIAAGGGITCNTTCAAG Henk and Vilgalys 2007
SEF1a1r2 CATICCGGCCTTGATNGTNCC
O. sinensis rDNA OsT-F* GTCAAGAAGCAAGCAAAGGAATC Zhong et al. 2014
OsT-R* TCAACTGGAGGGTGTGGTGG
MAT1-2-1 Mat1-2F* TGGAATGCGACTGACTACGA Zhang et al. 2011**
Mat1-2R* CCAGGAGAGCTTGCTTGACT
MAT1-1-1 MATF2 AAACGCCCTCTCAAYGCNTTYATG Bushley et al. 2013**
MATR3 CCACTTGCTTCTGAANGGRTCYTTRTTCCA
DNA lyase DNAF3 TTGATGGGATCCGAYCAYTGYCC
DNAR2 GTGACCAGGCTGATRCANGGYTC
MAT1-1-3 M3F1 CAGCAGCCGGTGAAGGTNTWYCAYGA
M3R1 CTTCGTTGTGTACTTGTANYCNGGRTA
β-Tubulin T1 AACATGCGTGAGATTGTAAGT Quan et al. 2014
T22 TCTGGATGTTGTTGGGAATCC
SSU rRNA NS1 GTAGTCATATGCTTGTCTC Zhong et al. 2010**
NS4 CTTCCGTCAATTCCTTTAAG
C. militaris MAT1-1-1 MAT1-F1 CGRGCWAARCGRCCATTKAAYGC Yokoyama et al. 2006**
MAT1- R1 TTKCCCATCTCRTCRCGGAYRAARGA
MAT1- 2-1 MAT2 -F1 GCRTATA TTCT RT ACCGC AG
MAT2-R1 CGAGGTT GATAYTGAT AYT G
T. indicum complex β-Tubulin Bt2a GGTAACCAAATCGGTGCTGCTTTC Wang et al. 2006a
Bt2b ACCCTCAGTGTAGTGACCCTTGGC
T. melanosporum MAT1-1-1 P19* CAATCTCACTCGTGATGTCTGGGTC Rubini et al. 2011b**
P20* TCTCGGGCTGGAGGTGCGGGTCGAGT
MAT1-2-1 P1* CAGGTCCGTCATCTCCTTCCAGCAG
P2* CCACATGCGACCGAGAATCTTGGCTA
Tuber truffles EF1α Tuber_f* AGCGTGAGCGTGGTATCAC Bonito et al. 2013**
Tuber_r* GAGACGTTCTTG ACGTTGAAG
RPB2 Tuber_f* YAAYCTGACYTTRGCYGTYAA
Tuber_r* CRGTTTCCTGYTCAATCTCA
PKC gene pkc1f CCCAAAGGTGGTCACGAAGTGTA Wang et al. 2006b**
pkc1r TGATGAACTCCTTCTTCAGAACC
β-Tubulin Bt1a TTCCCCCGTCTCCACTTCTTCATG
Bt1b GACGAGATCGTTCATGTTGAACTC

Note: *The special primers; **there are other primers for the target in the reference. The references of black body present available universal or special primers of ITS.