FIG 6 .

Overexpression of RcpA abolishes curli production in other E. coli strains. (A) CR staining of MS7163 and UTI89 in the absence and presence of plasmid pRcpA following growth on YESCA-CR agar. Strains were grown by spotting 5 µl of an overnight culture on YESCA-CR agar and incubating for 24 h at 37°C. (B) Western blot analysis of CsgA performed using whole-cell lysates prepared from MS7163 and UTI89 in the absence and presence of plasmid pRcpA. Bacteria were grown on YESCA agar for 24 h to induce curli production and treated with formic acid to dissolve polymerized CsgA. Anti-OmpA antibody was used as a loading control.