FIG 2 .

High-throughput screening identifies filamentation-competent mutants that are defective in activation of macrophage pyroptosis. (a) Images showing ASC-cerulean macrophages infected with C. albicans and stained with propidium iodide. Bar, 20 µm. The bar chart shows the percentage of cells positive for ASC specks only, propidium iodide staining only, or both. (b) We identified 98 genes that are required for wild-type levels of pyroptosis but that have no effect on filamentation. Functional clusters were defined by Gene Ontology (GO) term annotation. ER, endoplasmic reticulum.