FIG 5 .
The Thr residue in the P3 position of the 1/2 site of SINV slows down P123 processing but does not increase production of PAMP RNAs in infected BHK-21 cells. (a) Processing of SINVI538 and SINVT538 nonstructural polyproteins was analyzed as described in the legend to Fig. 3a. (b and c) BHK-21 cells were infected with SINVI538 or SINVT538 at an MOI of 10. At 4 or 6 h p.i., cells were lysed, and total RNA was isolated. (b) Northern blot analysis was performed as described in the legend to Fig. 4b except that probes revealing positive and negative strands of SINV RNAs were used. (c) One microgram of each RNA sample was used for transfection of Cop5 cells. The amount of IFN-β in the cell supernatant at 24 h p.t. was determined. IFN-β amounts are expressed as the means ± SEM from three experiments performed in parallel (*, P < 0.05 using Student’s two-tailed unpaired t test). Data from one of two reproducible independent experiments are shown in each of the panels.