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. 2018 May 25;2(7):765–778. doi: 10.1210/js.2018-00007

Figure 2.

Figure 2.

Notch signaling components and PGR expression in EU and ECs. (a) Paraffin-embedded eutopic endometrial tissue (n = 17) and ECs (n = 14) from women with diagnosed endometriosis (Taiwan cohort) were immunostained with anti-NICD1 antibody. Staining scores (0 = none, 1 = weak, 2 = moderate, and 3 = strong) were assigned to immunostained samples. H-scores (mean ± SD) were calculated as described under “Materials and Methods.” **P < 0.0001 by Student unpaired t test between stromal EU and ECs. (b) Stromal cells were isolated from paired EU and ECs of women with endometriosis (n = 6) and subjected to quantitative polymerase chain reaction analyses to quantify Notch signaling components HES1 and JAG2 mRNA levels. (c) Stromal cells were isolated from paired EU and ECs of women with endometriosis (n = 6) and subjected to quantitative polymerase chain reaction analyses to quantify total PGR and PGR-B mRNA levels. For b and c, data (mean ± SD) were normalized to 18S rRNA. *P < 0.05 by Student paired t test between stromal EU and ECs.