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. 2018 May 3;114(11):1499–1511. doi: 10.1093/cvr/cvy110

Figure 5.

Figure 5

β1-ARs and β2-ARs differentially regulate the PKA target gene, ICER in cardiomyocytes. (A) ICER mRNA expression in ARVMs transduced during 24 h with Ad.β-Gal (MOI 250) with or without β1-AR stimulation (100 nM Iso plus 10 nM ICI during 2 h), Ad. PKI-NES (MOI 250) with β1-AR stimulation (100 nM Iso plus 10 nM ICI during 2 h) and Ad.PKI-3NLS (MOI 250) with β1-AR stimulation (100 nM Iso plus 10 nM ICI during 2 h). (B) Effects of β1-AR stimulation (100 nM Iso plus 10 nM ICI during 2 h) with concomitant PDE4 inhibition (Ro, 10 µM) on ICER mRNA expression in ARVMs transduced during 24 h with Ad.β-Gal (MOI 250) or Ad.PKI-3NLS (MOI 250). (C) Effects of β2-AR stimulation (100 nM Iso plus 100 nM CGP during 2 h) with concomitant PDE4 inhibition (Ro, 10 µM) on ICER mRNA expression in ARVMs transduced during 24 h with Ad.β-Gal (MOI 250) or Ad.PKI-3NLS (MOI 250). (D) Effects of β2-AR stimulation (100 nM Iso plus 100 nM CGP during 2 h) with concomitant PDE4 inhibition (Ro, 10 µM) on ICER mRNA expression in ARVMs transduced during 24 h with Ad.β-Gal (MOI 250) and pre-treated or not with 10 µM CE3F4 during 15 min at 37°C. (E) Effects of β2-AR stimulation (100 nM Iso plus 100 nM CGP during 2 h) with concomitant PDE4 inhibition (Ro, 10 µM) on ICER mRNA expression in ARVMs transduced during 24 h with Ad.β-Gal (MOI 250) or Ad.PKI-NES (MOI 250). Number of rats is indicated in the respective panels. Statistical significance is indicated as **P < 0.01; ***P < 0.001 vs. β-Gal; $$$P < 0.001 by Kruskal-Wallis test with Dunn’s post-hoc test.