Fig. 1.

Primary cultures of microglia and hypothalamic neurons from rat brain. Primary cultures of microglia were obtained from rat cortex. (A) Cultured cells presented a high density of Iba-1 positive staining and very few GFAP positive staining. (B) Immunofluorescent stain analysis on that 94.2% cells presents Iba-1 positive staining, 5.5% of cells presents GFAP staining, and no NeuN positive staining was visualized. (C) Phase contrast views of the primary cultures of hypothalamic neuron culture on days 1, 3, 5 and 7 post-plating. (D) Immunocytochemistry staining revealed extensive CRF- and NeuN-positive staining in the primary cultured hypothalamic neurons on day 7. (E) A very low density of Iba-1 positive cells presented in cultured hypothalamic neurons. (F) Low and high resolution of cultured neurons. (G) Percentage of NeuN, Iba-1 and GFAP positive stainings was 73%, 6.7% and 14.3%, respectively. 6% cells did not show any positive staining. Scale bar = 100 μm.