Fig. 2.

LPS induces morphological change, increases cellular Iba-1 expression and IL-1β level in culture medium of BV2 cells. (A) BV2 cells without LPS treatment presented ramified cell morphology with fine processes extending tens of microns away from the soma (left). BV2 cells treated with LPS (10 μg/mL) presented a rounded amoeboid-like appearance with fine processes from soma (right). (B and C) LPS-treated BV2 cells present higher Iba-1-positive staining compared with the non LPS-treated BV2 cells (B, low magnification, and C, high magnification). LPS incubation induced IL-1β level in culture medium compare with non-LPS treated group (D). All data are presented as means ± SEM; **p < 0.01; Scale bar = 100 μm.