Fig. 1.

Relative activity of the secreted enzymes of C. stercorarium grown on oat spelt xylan (light grey) or filter paper (dark grey). Enzymatic activity was determined by DNSA-based assay. 5.0 mg/L extracellular enzymes, precipitated from the culture supernatant, were incubated with 0.5% (w/v) polysaccharide at pH 6.5 and 60 °C in 0.1 M MOPS reaction buffer overnight (16 h). Hydrolytic activity from oat spelt xylan medium on soluble wheat arabinoxylan was set to 100%. Tested polysaccharides with no activity were: arabinan, arabinogalactan (larch), curdlan, galactomannan (guar), gum arabic (acacia), inulin (dhalia tubers), laminarin, mannan, mannan (ivory nut), rhamnogalacturonan I, sinistrin