Table 3.
Hemicellulolytic activity of seventeen glycoside hydrolases on 29 different polysaccharides
| Enzymes | Specific activity (U/mg) | |||||||
|---|---|---|---|---|---|---|---|---|
| Arabinan | Arabinoxylan (wheat, soluble) | Arabinoxylan (wheat, insoluble) | 4-O-Methyl-glucuron-oxylan | Xylan (birch) | Xylan (oat spelt) | Xyloglucan | β-Glucan (barley) | |
| Bga2B | (+) | – | – | – | – | – | 3.11 ± 0.04 | – |
| Bxl3B | – | 0.25 ± 0.01 | + | 0.21 ± 0.01 | 0.28 ± 0.01 | 0.35 ± 0.02 | – | – |
| Cel9Z | – | – | – | (+) | – | (+) | – | 159 ± 1 |
| Xyn10E | – | 0.17 ± 0.01 | – | + | (+) | (+) | – | – |
| Xyn10C | – | 134 ± 5 | 14.1 ± 0.8 | 91.3 ± 3.8 | 56.3 ± 2.0 | 86.6 ± 1.2 | – | 19.1 ± 0.1 |
| Xyn10B | – | 597 ± 46 | 40.4 ± 3.7 | 483 ± 45 | 185 ± 20 | 679 ± 41 | – | 18.8 ± 1.6 |
| Xyn10D | – | 6.55 ± 0.09 | – | + | 1.24 ± 0.04 | 6.17 ± 0.08 | – | (+) |
| Xyn11A | – | 1030 ± 50 | 226 ± 47 | 936 ± 19 | 619 ± 7 | 937 ± 6 | – | – |
| Man26A | – | – | – | – | – | – | + | – |
| Bga35A | (+) | – | – | – | – | – | – | – |
| Axh43A | + | 41.5 ± 0.8 | (+) | (+) | – | + | – | – |
| Arf43A | – | (+) | – | (+) | (+) | (+) | – | – |
| Arf43C | 0.82 ± 0.06 | – | – | – | – | – | – | – |
| Abn43A | 79.5 ± 0.9 | – | – | – | – | – | – | – |
| Arf51B | 2.01 ± 0.03 | 0.32 ± 0.01 | – | – | – | (+) | – | – |
| Gal53A | + | – | – | – | – | – | – | – |
| Xyn105F | – | 0.22 ± 0.01 | – | + | + | + | – | – |
| Enzymes | Specific activity (U/mg) | |||||||
|---|---|---|---|---|---|---|---|---|
| Lichenin | Galactan (potato) | Galactan (lupin) | Pectic galactan (potato) | Pectic galactan (lupin) | Mannan | Galactomannan (guar) | Glucomannan (konjac) | |
| Bga2B | – | + | (+) | (+) | (+) | – | – | – |
| Bxl3B | – | – | – | – | – | – | – | – |
| Cel9Z | 136 ± 9 | – | – | – | – | – | – | 32.9 ± 1.0 |
| Xyn10E | – | – | – | – | – | – | – | – |
| Xyn10C | 7.85 ± 0.45 | – | – | – | (+) | – | – | + |
| Xyn10B | 9.65 ± 0.65 | – | – | – | (+) | – | – | (+) |
| Xyn10D | – | – | – | – | (+) | – | – | – |
| Xyn11A | – | – | – | – | – | – | – | – |
| Man26A | – | – | – | – | – | 39.9 ± 2.0 | 216 ± 5 | 144 ± 3 |
| Bga35A | – | 2.17 ± 0.03 | 1.24 ± 0.08 | 1.08 ± 0.01 | 1.14 ± 0.02 | – | – | – |
| Axh43A | – | – | – | – | (+) | – | – | – |
| Arf43A | – | – | – | – | (+) | – | – | – |
| Arf43C | – | – | 1.99 ± 0.20 | 6.50 ± 0.13 | 4.46 ± 0.09 | – | – | – |
| Abn43A | – | – | 10.1 ± 0.3 | 21.3 ± 0.2 | 12.0 ± 2.1 | – | – | – |
| Arf51B | – | – | – | (+) | – | – | – | – |
| Gal53A | – | + | + | + | + | + | – | – |
| Xyn105F | – | – | – | – | – | – | – | – |
Hydrolysis experiments were performed in 0.1 M MOPS reaction buffer with 0.05–24 mg/L enzyme at pH 6.0 (pH 6.5 for Abn43A) and the temperature optimum of each enzyme for incubation times from 30 min up to 2 h. The specific enzyme activity was determined using DNSA assay in triplicates. Further tested polysaccharides for which no activity could be determined were: arabinogalactan (larch), curdlan, gum arabic (acacia), inulin (dahlia tubers), laminarin, mannan (ivory nut), pachyman, polygalacturonic acid, pullulan, rhamnogalacturonan I, sinistrin, chitosan and Avicel. No activity (–), detectable but very low activity ((+)), activity overnight (+), specific activity (numbers)