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. 2018 Aug 22;37:200. doi: 10.1186/s13046-018-0867-3

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — IL-6 expression in human preadipocytes was increased by co-culture with human breast DCIS cells. a Experimental scheme illustrating the transwell culture of DCIS.com (DCIS.com) and human preadipocytes (hPreAd). b Measurement of secreted IL-6 expression in single or co-culture, as assessed by ELISA. Data are presented as the means ± standard errors of three independent experiments. c Analysis of IL-6 and IL-6 receptor (IL-6R) mRNA expression levels in DCIS.com and hPreAd in single culture (S) or co-culture (C), as assessed by RT-PCR. d Analysis of IL-6 and IL-6R protein expression levels in DCIS.com and hPreAd in single culture (S) or co-culture (C), as assessed by western blot. e Analysis of IL-6 and IL-6R protein expression levels in DCIS.com and hPreAd treated with each conditioned medium (CM), as assessed by western blot