Table 2.
Relative quantity (RQ) of GAL2 gene expression levels for cultivations of S. cerevisiae DS61180 under microaerobic conditions in shake flasks on the substrates d-glucose, d-galactose, and l-arabinose in minimal medium
| Substrates | RQ of GAL2 (%) |
|---|---|
| Single substrates | |
| d-Glucose (negative control) | 0.52 (± 0.04) |
| d-Galactose (positive control and reference) | 100.01 (± 3.71) |
| l-Arabinose | 21.29 (± 0.84) |
| Mixed substrates | |
| d-Glucose/l-arabinose mixture (growth on d-glucose in presence of l-arabinose) | 0.23 (± 0.05) |
| d-Glucose/l-arabinose mixture (growth on l-arabinose after d-glucose depletion) | 4.06 (± 0.22) |
Substrates were either provided as single substrates or as mixture of d-glucose and l-arabinose. SGA1 was used as endogenous control and the cultivation on d-galactose served as reference. Given errors indicate standard deviations from three measurements of biological duplicates (n = 6)