Table 5.

Concordance in direction of regulation of overlapping gene lists

A) Rich diet overlap between presence and absence of Nosema	PN vs CN	P vs C
		Total		Up	Down	Significance
		131	Up	7	0	X-squared = 85.458 p-value < 2.2e-16
			Down	2	122
	BN vs CN	B vs C
		Total		Up	Down	Significance
		11	Up	0	5	X-squared = 0.090909 p-value =0.763 *
			Down	0	6
	MN vs CN	M vs C
		Total		Up	Down	Significance
		21	Up	15	0	X-squared = 8.4077 p-value = 0.003736
			Down	2	4
B) Overlap between rich diets in healthy bees	P vs C	B vs C
		Total		Up	Down	Significance
		64	Up	5	0	X-squared = 50.86 p-value = 9.877e-13
			Down	0	59
	P vs C	M vs C
		Total		Up	Down	Significance
		56	Up	5	0	X-squared = 44.377 p-value = 2.708e-11
			Down	0	51
	B vs C	M vs C
		Total		Up	Down	Significance
		61	Up	22	0	X-squared = 56.74 p-value = 4.974e-14
			Down	0	39
C) Overlap between rich diets in Nosema infected bees	PN vs CN	BN vs CN
		Total		Up	Down	Significance
		117	Up	4	0	X-squared =88.674 p-value = <  2.2e-16
			Down	0	113
	PN vs CN	MN vs CN
		Total		Up	Down	Significance
		90	Up	0	0	X-squared = 67.6 p-value < 2.2e-16
			Down	6	84
		MN vs CN
	BN vs CN	Total		Up	Down	Significance
		44	Up	1	0	X-squared =10.494 p-value =0.001197
			Down	0	43

Overlapping genes are sorted according to direction of regulation. PN vs. CN, BN vs. CN and MN vs. CN are, respectively, pollen, Bee-Pro and MegaBee diet treatments with Nosema. P vs. C, B vs. C, and M vs. C are, respectively, the same diet treatments in healthy bees. Chi-square tests with Yates correction were performed to determine if pattern of direction bias is statistically consistent between the considered two conditions. Asterisk (*) indicates lack of pattern consistence