Fig. 8.

Representative images of immunofluorescence labeling of Rhcg and cell type-specific markers in brain tissues (× 400 original magnification). a Double-immunofluorescence analysis of Rhcg (red) and CD31 (green). b Double-immunofluorescence analysis of Rhcg (red) and GFAP (green). c Double-immunofluorescence analysis of Rhcg (red) and NeuN (green). Rhcg was detected in BMECs and astrocytes and at a lower level in neurons. N.S. normal saline, D-GalN, d-galactosamine hydrochloride, LPS lipopolysaccharide, TNF-α tumor necrosis factor-alpha, BMECs brain microvascular endothelial cells, GFAP glial fibrillary acidic protein, ALF acute liver failure, DAPI _{4',6-diamidino-2-phenylindole}