Table 3.
Population doubling level of disc cells cultured in different culture media
| PD | alpha-MEM | DMEM | Ham’s F-12 | alpha/F-12 | DMEM/alpha | DMEM/F-12 | |
|---|---|---|---|---|---|---|---|
| AF | P0 | 3.5 ± 0.1 | 3.3 ± 0.4 | 2.9 ± 0.1 | 3.3 ± 0.1 | 3.5 ± 0.3 | 3.4 ± 0.2 |
| P1 | 3.3 ± 0.4 | 3.1 ± 0.8 | 2.6 ± 0.6 | 2.6 ± 0.4 | 3.0 ± 0.5 | 2.8 ± 0.6 | |
| P2 | 3.3 ± 0.5 | 3.1 ± 0.0 | 3.1 ± 0.6 | 3.1 ± 0.6 | 3.4 ± 0.3 | 3.1 ± 0.6 | |
| cPDL | 10.2 ± 0.2 *# | 9.5 ± 0.4 | 8.5 ± 0.8 | 9.0 ± 0.7 | 9.9 ± 0.7 * | 9.3 ± 0.7 | |
| NP | P0 | 3.7 ± 0.4 | 3.3 ± 0.5 | 3.1 ± 0.4 | 2.9 ± 0.2 | 2.7 ± 0.7 | 2.8 ± 0.5 |
| P1 | 3.0 ± 0.1 | 2.8 ± 0.3 | 2.5 ± 0.3 | 2.5 ± 0.4 | 2.7 ± 0.2 | 2.8 ± 0.0 | |
| P2 | 2.9 ± 0.2 | 2.9 ± 0.2 | 2.1 ± 0.3 | 2.5 ± 0.2 | 2.8 ± 0.3 | 2.8 ± 0.3 | |
| cPDL | 9.6 ± 0.3*#+§ | 9.1 ± 0.6*# | 7.7 ± 0.5 | 7.8 ± 0.6 | 8.2 ± 0.2 | 8.3 ± 0.5 | |
PD Population doubling in each passage P0, P1 and P2; cPDL cumulated PD level at the end of passage P2; AF annulus fibrosus; NP nucleus pulposus. Data is shown as mean ± SD (AF n = 5, NP n = 3).
*Significant (p value < 0.05) compared to Ham’s F-12
#Significant (p value < 0.05) compared to alpha/F-12
+Significant (p value < 0.05) compared to DMEM/alpha
§Significant (p value < 0.05) compared to DMEM/F-12