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. 2018 Aug 9;9:649. doi: 10.3389/fneur.2018.00649

Figure 4.

Figure 4

Expression of three types of ERs in hippocampus. (A) Immunofluorescence staining in hippocampal CA1 and CA3 regions (boxed region in B) showing ERs (red), neurons (green), and nuclei stained with DAPI (blue). Scale bar = 50 μm. (B) A portion of one side of the hippocampus marked by NeuN. Scale bar = 200 μm. (C–H) The number of ER immunoreactive neurons and neurons in the hippocampal CA1 and CA3 regions; n = 4; *P < 0.05. (I–K) Colocalization of ERs with NeuN at higher-magnification views. Scale bar = 20 μm. ER, estrogen receptor; GPR30, G protein-coupled estrogen receptor.