Figure 2.

CAR overexpression suppresses TNF-α induced inflammation in human colon cell lines. NCM460 and SW480 cells were transfected with CAR overexpression plasmid and treated with control or TNF-α; cells were then harvested for qPCR detection and Western-blot assay. (a & b) CAR mRNA level were detected by qRT-PCR in CAR overexpression human colon cells NCM460 (a) and SW480 (b). (c–f) mRNA levels of inflammatory genes IL-21 (c & d) and IL-23 (e & f) expression upon CAR overexpression. (g) Western-blot examination of protein levels of inflammatory gene P65 and cell adhesive gene upon TNF-α and CAR overexpression in human colon cells lines. β-Actin levels of each sample were used as endogenous loading control. (h) Quantitative analysis of P65 protein levels (calibrated with β-actin levels of each sample) in (g). All data represent at least 3 independent experiments. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001; data represent mean ± s.e.m.