Figure 6.

Effects of CAR overexpression on DSS induced inflammation in vivo. (a & b) Expressions of inflammatory genes IL-21 and IL-23 were determined by qPCR. Colon tissue samples were collected after animals were treated with control or DSS/DSS+CAR, mRNA was isolated, and genes were detected by specific primers. (c) Protein levels of tight junction gene P65 were determined by Western blot using specific antibodies, and β-actin levels were used as endogenous loading control. (d) Quantitative analysis of P65 protein levels (calibrated with β-actin levels of each sample) in (c). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001; data represent mean ± s.e.m. from 3 independent experiments with sample size = 3 for each group.