Figure 7.

CAR protects mice from DSS induced colon permeabilization in vivo. (a) FITC-Dextran permeability assay measurement of colon permeability after different treatment. (b & c) Expression of tight junction gene ZO-1 (a) and Occludin (b) were determined by qPCR. Colon tissue samples were collected after animals were treated with control or DSS/DSS+CAR, mRNA was isolated, and genes were detected by specific primers. (d) Protein levels of CAR and tight junction gene ZO-1 and Occludin were determined by Western blot using specific antibodies, and β-actin levels were used as endogenous loading control. (e) Quantitative analysis of CAR, ZO-1, and Occludin protein levels (calibrated with β-actin levels of each sample) in (d). (f) Immunohistochemistry staining detection of ZO-1 levels in the colon tissues of different treatment groups. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001; data represent mean ± s.e.m. from 3 independent experiments with sample size = 3 for each group.