Figure 4.

AECK extract induces apoptosis in both extrinsic and intrinsic pathways. (a) Antibodies Bcl-2 and (b) Fas were utilized to incubated with U937 cells and then treated with FITC-conjugated anti-rabbit IgG, analyzed with flow cytometry; (c) U937 was seeded on cells climbing, treated with 64 μg/ml AECK and 2 μg/ml cordycepin for various lengths of time, And then analyzed by confocal fluorescent microscopy after stained with Fluo-3AM. (d) The line chart of Ca2+ density variation according to indicated time.