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. 2018 Aug 8;7:47. doi: 10.1038/s41377-018-0048-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Confocal images of live HeLa cells after photoporation with SNAP-Surface® Alexa Fluor® 647 (gray; left). Cells were first transfected with SNAP^LaminA or SNAP^Barttin. The nuclei are stained with Hoechst (cyan; middle). The right column are merged images. c, d Similarly, transfected and labeled live cells were imaged by Airyscan super-resolution microscopy. The right column are merged images. e Time-lapse TIRF microscopy images of Barttin-labeled HeLa cells. The yellow arrowhead indicates vesicle-mediated transport of Barttin. Scale bars are 10 µm