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. 2018 May 30;7:10. doi: 10.1038/s41377-018-0003-3

Fig. 5. Multimodal nonlinear endoscopy in biological tissues.

Fig. 5

a CARS image of fresh fatty tissues obtained from human colon. b SHG image of rat tail tendons featuring collagen fibers. c Close-up view of b confirming the 0.8 µm lateral resolution for nonlinear imaging. d, e Multimodal SHG (green, d1, e1) and CARS (red, d2, e2) images of fresh human colon tissues taken 50 µm below the sample surface. The large images are overlaps of the SHG and CARS images. SHG images required higher powers and were acquired immediately after taking the CARS images. Laser powers incident on the sample: CARS pump 20 mW, Stokes 10 mW; SHG 60 mW (45 mW for images b and c). Excitation wavelengths: 800 nm (pump CARS, SHG) and 1040 nm (Stokes). All images were averaged over five acquisitions for a total acquisition time of 6.5 s, except b and c that were averaged 10 times (13 s). The color scale in (counts per second) is provided on Fig. S14