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. 2018 Aug 23;13(8):e0201690. doi: 10.1371/journal.pone.0201690

Fig 1. Proteins associated with Csde1 upon pull down of biotagged Csde1.

Fig 1

(A) Lysate of MEL cells expressing the biotin ligase BirA with or without biotagged Csde1 was incubated with streptavidin beads, washed, and eluted in laemmli buffer before loading on SDS-PAGE and silver staining. Third lane is a protein size ladder, sizes indicated in kD next to the lane. (B) Mass spectrometry of proteins pulled down by Csde1, and analysis by two-way t-test revealed 8 Csde1-associated proteins at a significance threshold of S0 = 0.8. -10log p-value is plotted against 2log fold change (n = 3). (C) Protein profile expression plot of Csde1-associated proteins (LFQ: Label free quantification). Lines are discontinued when peptides were not detected in control pull down in BirA only MEL cells.