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. 2018 Aug 23;13(8):e0201690. doi: 10.1371/journal.pone.0201690

Fig 2. Knockdown of Strap does not affect the pool of Csde1-bound transcripts.

Fig 2

(A) MEL cells expressing BirA and biotagged Csde1 were treated with a short hairpin targeting Strap or a non-targeting short hairpin (Sc). Knockdown of Strap was confirmed by Western blot staining for Strap and Stat5 as a loading control. (B) Principal component analysis of transcripts bound to streptavidin beads incubated with MEL cells expressing BirA with or without biotagged-Csde1 and treated with anti-Strap shRNA (KD Strap) or control shRNA (Sc). Three biologically independent experiments were performed (a-c) (C) Venn diagram showing the overlap between Csde1-bound transcripts detected at a FDR<0.05 (biotag-Csde1 plus BirA versus BirA only) in previous experiments in MEL cells [15] and in MEL cells expressing control shRNA (Sc) or shRNA against Strap (KD Strap) (D) The enrichment of transcripts on streptavidin beads (biotag-Csde1 + BirA versus BirA only) compared between MEL cells expressing control shRNA (Sc) or shRNA against Strap (KD Strap) plotted for the combined transcripts as shown in (C). (C/D) red numbers/dots detected in parental MEL and in shRNA treated MEL; blue numbers/dots detected in previous study with parental MEL; green numbers/dots detected in shRNA treated MEL.