Fig 3. Strap knockdown affects the mRNA expression of select Csde1-bound transcripts.

MEL cells were treated with control and anti-Strap shRNA in 3 independent experiments. Cells were processed for transcriptome analysis by RNA sequencing. (A/B) The fold change expression of transcripts in Strap shRNA versus Sc shRNA treated MEL cells (2log values; X-axes) was compared to the likelihood of that transcript being bound to Csde1 in Sc treated MEL cells (likelihood enrichment on streptavidin beads in Sc treated MEL cells expressing biotagged Csde1 plus BirA compared to the SC treated MEL cells expressing BirA only; -10log values; Y axes) (A), or to the fold-change enrichment of that transcripts on streptavidin beads incubated with lysate of Sc treated MEL cells (B). All transcripts selected as in Fig 2C and 2D are included. Transcripts indicated by closed symbols are differentially expressed in Sc and anti-Strap treated MEL cells at FDR<0.05. Blue triangles represent transcripts previously reported as Csde1-bound transcripts in MEL cells with FDR <0.05; green diamonds are transcripts only associated with Csde1 at FDR<0.05 in shRNA treated MEL cells; red circles were detected at a FDR<0.05 in both shRNA treated and non-treated MEL cells.