Fig 3. 15-HETE levels in cardiac fibroblasts under different oxygen tensions and ALOX15 inhibition.

Cardiac fibroblasts isolated from left (LA) and right (RA) atrium as well as left (LV) and right (RV) ventricle were subjected to 1% or 21% oxygen for 24 h. Hypoxic fibroblasts were cultured with or without the specific ALOX15 inhibitor ML351 (10 μmol/L) or the less specific ALOX12 and ALOX15 inhibitor baicalein (10 μmol/L). n = 5 for every chamber in the hypoxic and normoxic groups. n = 3 for every chamber in the inhibitor groups, except for ML351 treated fibroblasts from right atrium (n = 2). Two-way ANOVA was performed with Tukey's multiple comparisons test using log-transformed data. Concentrations were then normalized by subtracting the normoxic controls and means were calculated for demonstration. * p < 0.05, ** p < 0.001.