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. 2018 Aug 23;13(8):e0202677. doi: 10.1371/journal.pone.0202677

Fig 5. The effects of SMOX induction by curcumin on DNA damage and growth inhibition.

Fig 5

(A) AGS cells were treated for 48 h with curcumin in the presence or absence of 250 μM MDL72527, a polyamine oxidase inhibitor. (B) SMOX knockout or control AGS cells were treated for 48 h with increasing concentrations of curcumin. Lysates were analyzed for changes in γH2AX by Western blot. (C) MTS assays were conducted with the same conditions as those in (A) and (B) to determine the effect of SMOX on curcumin-induced growth inhibition. (D) Intracellular spermine (SPM) and spermidine (SPD) levels of SMOX KO cells lines following curcumin exposure for 48 h. Data points indicate the means; error bars represent SEM; n = 3. CT1 and CT2 = CRISPR control AGS cell lines.