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. 2018 Aug 23;13(8):e0202797. doi: 10.1371/journal.pone.0202797

Fig 1. Silencing of YKL-40 in RAW264.7 cells by lentiviral vector-mediated YKL-40 shRNAs.

Fig 1

RAW264.7 cells were transduced with 50 MOI of 4 different kinds of shRNA vector, and YKL-40 expression was measured on day 4 by real-time PCR following transduction. GAPDH was used as an internal control. (A) YKL-40 mRNA expression was detected by real-time PCR (n = 8, *P<0.05). (B) RNAi inhibited the expressions of MCP-1 in RAW264.7 cells (n = 8). (C) RNAi inhibited the expression of MMP-8 in RAW264.7 cells (n = 8). No significant difference was found between the vehicle and scrambled groups. Vehicle = vehicle group; Scrambled = scrambled group (negative control). “-” and “+” indicate the absence and presence of lentivirus, respectively. Data are shown as the mean values ± SD obtained from triplicate experiments. *P<0.05 vs. vehicle groups; ▲P>0.05 vs. vehicle group.