Fig 2. Assessment of apoptotic/necrotic effects of different concentrations of SIM administered alone or in combination with 100 μM DMXAA on the co-culture model.

Double labeling for apoptosis (Annexin V-FITC) as well as for necrosis (PI) was performed. Relative fluorescence units measured were normalized for the number of cells in each condition. Data are shown as percentages of apoptosis (A) or necrosis (B) in comparison with positive controls and represented as mean ± SD of two independent measurements. SIM: cells incubated with different concentrations of SIM; SIM+100 μM DMXAA: cells incubated with different concentrations of SIM administered in combination with 100 μM DMXAA.