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. 2018 Aug 23;13(8):e0202827. doi: 10.1371/journal.pone.0202827

Fig 8. The effects of the combined treatment on macrophage polarization.

Fig 8

(A) Effects of SIM and DMXAA co-administration on the levels of ARG-1 and IL-10 mRNA in IL-4-treated BMDMs under hypoxia-mimicking conditions. mRNA was quantified by RT-qPCR and the results are expressed as fold change based on the Ct calculations. Untreated cells were used as calibrator. The results are expressed as mean ± SD of three independent measurements (B) Nitrite levels in lysates from IL-4-treated BMDMs incubated with SIM co-administered with DMXAA under hypoxia-mimicking conditions. The results are expressed as mean ± SD of two independent measurements. Control = untreated IL-4 polarized macrophages; SIM+DMXAA = IL-4 polarized macrophages treated with 3.5 μM SIM and 100 μM DMXAA (ns, P>0.05; ***, P<0.001).