Table 2. The effects of SIM and DMXAA administered as single as well as combined treatment on the pro-angiogenic protein production of the co-culture of B16.F10 cells and TAMs.
| Pro-angiogenic proteins | Percentage of inhibition (-) and stimulation (+) of pro-angiogenic protein production after different treatments | ||
|---|---|---|---|
| DMXAA | SIM | DMXAA+SIM | |
| G-CSF | +183±84 (***) | -42±4 (ns) | -59±8 (*) |
| GM-CSF | +241±126 (***) | +63± 6 (*) | -36±0.5 (ns) |
| M-CSF | +23±0.3 (ns) | -32±4 (ns) | -61±2 (*) |
| IGF-2 | +58±2 (*) | -2±3 (ns) | -57±0.7 (*) |
| IL-1ɑ | +91±8 (***) | +29±0.9 (ns) | -48±6 (ns) |
| IL-1ß | -34±12 (ns) | -81±0.1 (***) | -83±1 (***) |
| IL-6 | -1±42 (ns) | -32±8 (ns) | -62±5 (*) |
| IL-9 | +43±6 (ns) | -38±1 (ns) | -74±6 (**) |
| IL-12p40 | +20±9 (ns) | -41±1 (ns) | -46±0.5 (ns) |
| IL-13 | +91±4 (***) | +40±15 (ns) | +20±6 (ns) |
| TNF-α | -68±1 (**) | -78±1 (***) | -82±1 (***) |
| MCP-1 | +5±2 (ns) | -53±4 (ns) | -68±2 (**) |
| Eotaxin | -8±3 (ns) | -64±3 (*) | -94±2 (***) |
| FasL | +57±2 (*) | -63±1 (*) | -75±1 (**) |
| bFGF | -13±4 (ns) | -63±1 (*) | -84±2 (***) |
| VEGF | -54±0.3 (ns) | -71±18 (**) | -93±2 (***) |
| Leptin | -16±6 (ns) | -39±7 (ns) | -90±0.4 (***) |
| Thrombopoietin | -26±4 (ns) | -75±4 (**) | -87±1 (***) |
The pro-angiogenic protein levels in cell lysates after different treatments are compared to control levels of the same proteins. The results are expressed as % of the average inhibition (-) or stimulation (+) ± SD of two independent measurements. The two-way ANOVA Multiple Comparison Test was used to compare overall effects of different treatments on the production of these proteins with the levels of the same proteins in controls; DMXAA: cell co-culture treated with 100 μM DMXAA; SIM: cell co-culture treated with 3.5 μM SIM; DMXAA+SIM: cell co-culture treated with 100 μM DMXAA and 3.5 μM SIM. (ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001).