Table 1. Comparison of DNA extraction methods.
Extraction Method | Manual Time (hr) |
Automated Time (hr) |
A260/A280 mean ± s.d. |
A260/A230 mean ± s.d. |
Amplification |
---|---|---|---|---|---|
QIAmp® PowerFecal® | 1.5–2 | N/A | 1.84 ± 0.02 | 1.68 ± 0.23 | 40/40 |
Maxwell RSC PureFood GMA Authentication | 0.5 | 0.6 | 2.04 ± 0.03 | 1.86 ± 0.15 | 39/40 |
Time of extraction method determined from start of fecal processing to DNA elution and separated into manual and automated components. Mean 260/280 nm and 260/230 nm absorbance ratios (± s.d.) for each extraction method. With respect to more stringent filtering associated with DADA2, successful amplification was defined as minimal coverage of 100 reads per sample. n = 40 per extraction method.