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. 2018 Aug 9;7:e36654. doi: 10.7554/eLife.36654

Figure 4. Domain analysis of Swc5 using H2A.Z VivosX.

Figure 4.

(A) The cartoon depicts the domain organization of yeast Swc5 (Sun and Luk, 2017). (B,C) The yeast strain, HTZ1(T46C)FL swc5∆, was transformed by a single-copy plasmid containing either the wild-type SWC5 or the indicated SWC5 mutants or by the control vector (swc5∆). Top panels: Each strain was represented by three independent transformants and analyzed by VivosX in parallel. Htz1(T46C)FL and its crosslinked (ZxZ) adducts was detected by anti-FLAG immunoblotting. Bottom panels: Quantification of the immunoblots above. Bars and error bars represent the means and standard deviations of three biological replicates.