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. 2018 May 14;37(34):4692–4710. doi: 10.1038/s41388-018-0273-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — KMT2C loss suppresses ERα target gene expression. a Supervised analysis of the 7938 differentially expressed genes between shRenilla and shKMT2C (shKMT2C#1 and #2 combined) MCF7 cells. All cells are cultured in full serum containing media. b GSEA showing 3857 genes downregulated in shKMT2C cells are enriched among genes downregulated following 5-day estrogen deprivation of shRenilla cells (≥3-fold). c GSEA of 3857 genes downregulated in shKMT2C as compared to the Hallmark Estrogen Response Early Geneset (Broad Institute). d GSEA of 3857 genes downregulated in shKMT2C as compared the Hallmark Estrogen Response Late Geneset. e mRNA levels; values correspond to the mean of three replicates ± s.e.m. E2, estradiol. f mRNA levels; values correspond to the mean of three replicates ± s.e.m.; two-tailed Student’s t-test with a desired FDR = 1% was used to determine statistical significance; **P < 0.01, ***P < 0.001, ****P < 0.0001. Data correspond to one representative assay from a total of two or three independent assays. g Immunoblot; β-actin used as loading control. h mRNA levels, as measured by qRT-PCR, from RNA taken from the mammary glands of 12-week old virgin females. Values correspond to the mean of three replicates ± s.e.m. i Representative slides of PR expression in mammary glands from 12-week-old virgin females