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. 2018 Aug 23;8:12708. doi: 10.1038/s41598-018-31137-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SFN promotes the expression of the Slc40a1 iron importer in brain vascular endothelial cells. SFN promotes the transcription of the Slc40a1 iron exporter gene (fold change) as shown by MitoChip array (A1) and RT-PCR analysis (A2). Results were further confirmed through measurements of Slc40a1 protein expression by western blots (B1) and immunofluorescence (B2). Image scale = 100 µm at 40x magnification. N = 3–4 biological replicates per condition (assayed in triplicates). **p < 0.01, ***p < 0.001 and ****p < 0.0001, vs. controls. Blots of Slc40a1 and β-actin were taken from different gels.