Table 1.
Screening of Lactobacillus and Enterococcus strains for in-vitro probiotic attributes.
| # | Isolates | Acid tolerance (%) | Bile tolerance (%) | Simulated gastric digestion tolerance (%) | Simulated intestinal fluid tolerance (%) | Surface hydrophobicity (%) | Adherence (%) |
|---|---|---|---|---|---|---|---|
| 1 | L. paracasei D3-1 | 99.9 ± 3.5 | 101.4 ± 1.0 | 97.3 ± 4.7 | 54.4 ± 6.5 | 14.2 ± 0.2 | 20.3 ± 4.8 |
| 2 | L. paracasei D3-2 | 102.4 ± 5.3 | 103.3 ± 0.7 | 97.3 ± 5.3 | 54.6 ± 1.8 | 25.9 ± 0.2 | 20.4 ± 3.3 |
| 3 | L. paracasei D3-5 | 102.9 ± 2.2 | 100.7 ± 1.4 | 100.1 ± 0.8 | 57.6 ± 2.5 | 29.6 ± 0.8 | 26.3 ± 3.2 |
| 4 | L. rhamnosus D4-4 | 96.6 ± 3.0 | 101.5 ± 0.4 | 95.1 ± 3.5 | 54.9 ± 2.8 | 13.4 ± 0.0 | 14.7 ± 3.9 |
| 5 | L. plantarum D6-2 | 101.9 ± 5.4 | 102.2 ± 1.6 | 102.9 ± 8.7 | 58.6 ± 3.8 | 10.5 ± 0.1 | 32.4 ± 3.6 |
| 6 | L. rhamnosus D7-5 | 100.6 ± 1.7 | 101.8 ± 0.8 | 95.8 ± 0.7 | 54.2 ± 4.5 | 33.8 ± 0.3 | 15.5 ± 7.9 |
| 7 | L. rhamnosus D9-3 | 98.0 ± 3.2 | 101.2 ± 2.4 | 97.2 ± 6.2 | 49.9 ± 4.6 | 11.2 ± 0.7 | 21.9 ± 9.8 |
| 8 | L. rhamnosus D12-6 | 99.6 ± 5.3 | 94.5 ± 2.9 | 90.9 ± 10.7 | 50.8 ± 4.1 | 29.8 ± 1.4 | 17.4 ± 10.4 |
| 9 | L. plantarum D13-4 | 101.7 ± 4.9 | 103.3 ± 2.0 | 98.9 ± 0.9 | 50.2 ± 6.5 | 6.3 ± 0.0 | 25.8 ± 3.8 |
| 10 | E. raffinosus D24-1 | 64.0 ± 2.3 | 38.5 ± 0.3 | 0.4 ± 0.0 | 11.0 ± 0.7 | 13.6 ± 0.6 | 15.0 ± 0.4 |
| 11 | E. INBio D24-2 | 72.4 ± 5.5 | 60 ± 0.5 | 0.4 ± 0.0 | 7.3 ± 0.5 | 46.9 ± 0.9 | 5.1 ± 0.1 |
| 12 | E. avium D25-1 | 27.1 ± 1.2 | 72.5 ± 2.5 | 5.6 ± 0.4 | 13.2 ± 0.7 | 13.7 ± 0.7 | 9.0 ± 0.2 |
| 13 | E. avium D25-2 | 6.3 ± 0.1 | 44.7 ± 0.3 | 8.4 ± 0.5 | 44.8 ± 0.1 | 15.6 ± 0.8 | 9.0 ± 0.5 |
| 14 | E. avium D26-1 | 8.9 ± 0.1 | 58.4 ± 1.2 | 4.2 ± 0.2 | 25.5 ± 1.7 | 11.7 ± 0.8 | 44.2 ± 2.9 |
| 15 | E. gallinarum D21-6 | 0.1 ± 0.0 | 86.4 ± 3.1 | 0.3 ± 0.0 | 84.7 ± 2.4 | 1.0 ± 0.4 | 1.0 ± 0.0 |
| 16 | E. raffinosus D25-3 | 5.7 ± 0.4 | 63.2 ± 2.8 | 5 ± 0.6 | 63.0 ± 0.2 | 35.2 ± 0.5 | 13.7 ± 0.9 |