Figure 2.

Cell survival and vinculin assembly are influenced by switching the substrate elasticity. (A) Percentage of surviving SW480 cells based on the S1 from three pooled independent experiments (error bars represent s.e.m.). (B) Representative images of cells cultured for 24 h on glass, 1^st E₂₀, 2^nd E₂₀ and 3^rd E₂₀ immunolabelled with anti-vinculin counterstained with Hoechst 33258 and phalloidin. Scale bars: 10 µm. (C) Quantitative data for cell areas (in µm²), based on B, were determined using ImageJ. We used 200–300 cells for each condition (glass, 1^st E₂₀, 2^nd E₂₀ and 3^rd E₂₀). (D) Quantitative data for nuclear areas (in µm²) based on B were determined using ImageJ. We used 200–300 cells for each condition. (E) Quantification of vinculin spots (in µm) based on B by measuring 20 contacts per cell in 20 cells for each condition. (F) Quantification of actin stress fibers (in µm) using ImageJ based on B by measuring the length of 20 fibers per cell in 20 cells for each condition. (C–F) Results for three independent experiments are shown (error bars represent s.e.m.).