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. 2018 Aug 23;8:12692. doi: 10.1038/s41598-018-31179-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTPN22 is dispensable for FcγR dependent immune complex binding, uptake and processing. (a) WT (white) or Ptpn22^−/− (black) BMDCs were incubated with ova-AF488 ICs for 1 hour on ice. Cell surface ova-AF488 IC binding was determined by flow cytometry, gating on live, CD11c⁺, anti-rabbit⁺ singlets. n = 3–5 + s.d. (b) Combined ova-AF488 IC internalisation by WT (white) and Ptpn22^−/− (black) BMDCs was determined by flow cytometry, gating on live, CD11c⁺, anti-rabbit^- singlets. n = 3–5 ± s.d. (c) Representative flow cytometry plots showing ova-AF594:anti-ova coated bead internalisation and ova degradation by WT BMDCs over 0–7 hours at 37 °C. (d) Proportion of beads internalised by WT (white) and Ptpn22^−/− (black) BMDCs that have degraded ova-AF594 on them (gated on single, anti-rabbit^- beads). n = 3 ± s.d. (technical repeats, representative of 8 independent experiments). (e) Ova-AF594 geometric mean fluorescence intensity (GMFI) of beads internalised by WT (white) and Ptpn22^−/− (black) BMDCs (gated on single, anti-rabbit^- beads). n = 3 ± s.d. (technical repeats, representative of 8 independent experiments).