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. 2018 Aug 23;8:12700. doi: 10.1038/s41598-018-31058-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molar ratio of total sites grafted on the ns-nHA surface using different amino acids (AA) following the fluoraldehyde protocol. Aspartic acid was again found to attach to fewer available graft sites on ns-nHA than the other two amino acids. Data reported as average ± one standard deviation (n = 5). ns-nHA unsaturated with calcium is given as US, while that pre-saturated with calcium is given as CaS. Horizontal bars indicate statistically detectable difference (p < 0.05).