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. 2018 Aug 24;7(8):65. doi: 10.1038/s41389-018-0073-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a HCC1954 cells were transfected with scrambled (SCR), HAS3 (siHAS3), or CD44 siRNA (siCD44) oligos for 48 h and then the efficiency of HAS3 or CD44 silencing has been measured by qRT-PCR. siRNA oligos against CD44 mRNA was purchased by Qiagen (Flexitube siRNA SI03098123). Bars represent the mean of three independent experiments (n = 3, biological replicates) ± SD. No data were excluded from the analysis. *p-value < 0.05. **p-value < 0.01. b Representative images of the mammospheres derived from HCC1954 cells transfected as in a. c HCC1954 cells transfected as in a were plated for the sphere-forming assay. The sphere-forming efficiency (SFE) was calculated as the percentage of the number of spheres per plated cells. Values represent the mean of the number of spheres counted in 12 (n = 12) different fields. The values are representative of three independent experiments (n = 3 biological replicates). *p-value < 0.05. d HCC1954 cells were plated for the sphere-forming assay in the presence of the indicated concentration of 4-MU. SFE was calculated as in c. Values represent the mean of the number of spheres counted in 10 (n = 10) different fields. The values are representative of three independent experiments (n = 3 biological replicates). **p-value < 0.01.