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. 2018 Aug 23;9:3390. doi: 10.1038/s41467-018-05906-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — pHe and ATRA dual-enhanced cellular uptake and gene silencing. a Flow cytometry analysis of PSCs after incubation with Cy5-siRNA-loaded Au@PP or Au@PP/RA at pH 7.4 or 6.5 for 2 h. b The mean fluorescence intensity (MFI) of the different groups. The data are shown as the mean ± s.d. (n = 3). ^**p < 0.01, ^***p < 0.001 (Student’s t test). c Confocal laser scanning microscopy images of PSCs after incubation with Cy5-siRNA (red) loaded formulations at pH 7.4 or 6.5 for 2 h. F-actin was labelled with phalloidin (green) and nuclei were labelled with Hoechst 33342 (blue). Scale bars, 20 μm. d Western blot analysis of HSP47 protein in PSCs after treatment with the indicated formulations for 48 h at pH 7.4 or 6.5. Lipofectamine 2000 transfection agent served as a positive control (Lipo2000/siHSP47). siN.C, negative control siRNA; siHSP47, HSP47 siRNA. e Quantitative analysis of the normalised HSP47 protein expression (using Image J software). The data are shown as the mean ± s.d. (n = 3). ^**p < 0.01, ^***p < 0.001 (Student’s t test)