Fig. 2.

HSV-1 recruits ESCRT-III via the viral NEC. a Confocal microscope images of HeLa/CHMP4B-EGFP cells infected with wild-type HSV-1 or HSV-1 ΔUL34. Bars, 20 μm. Images are representative of three independent experiments. b Colocalization between CHMP4B-EGFP and lamin A/C in the experiment in (a) was quantified using Mander’s colocalization coefficient. Data are shown as the mean ± SEM (n = 21 for wild-type HSV-1 or 26 for HSV-1 ΔUL34-infected cells and are representative of three independent experiments). c Confocal microscope images of HeLa/CHMP4B-EGFP cells infected with wild-type HSV-1 or HSV-1 ΔUL31. Bars, 20 μm. Images are representative of three independent experiments. d Colocalization between CHMP4B-EGFP and lamin A/C was quantified using Mander’s colocalization coefficient in the experiment in (c). Data are shown as the mean ± SEM (n = 20 for wild-type HSV-1 or 21 for HSV-1 ΔUL31-infected cells in representative of three independent experiments). e Confocal microscope images of HeLa/CHMP4B-EGFP cells transfected with the UL31 and UL34 expression vectors. Bars, 10 μm. Images are representative of three independent experiments. The indicated P-values were obtained using the unpaired Student’s t-test (b, d)