Fig. 5.

ALIX is required for the recruitment of ESCRT-III to the NM in HSV-1-infected cells. a Confocal microscope images of HeLa/CHMP4B-EGFP cells treated with (left) control siRNA (siCt), (middle) siRNA to ALIX (siALIX), or (right) siRNA to CHMP7 (siCHMP7) for 48 h and subsequently infected with HSV-1 for 22 h. Bars, 20 μm. Images are representative of three independent experiments. b Colocalization between CHMP4B-EGFP and UL34 in the experiment in (a) was quantified using Mander’s colocalization coefficient. Data are shown as the mean ± SEM (n = 26 for siCt, 29 for siALIX and 23 for siCHMP7, and are representative of three independent experiments). The indicated P-values were obtained using the Tukey’s test. n.s., not significant