Fig. 6.

Effects of CHMP7 depletion on HSV-1 replication. a Confocal microscope images of HeLa cells treated with control siRNA (siCt), siRNA to CHMP7 (siCHMP7), or siRNA to CHMP4A, B, and C (siCHMP4ABC) for 48 h and infected with HSV-1 for 22 h. Images are representative of three independent experiments. Bars, 20 μm. b The percent of cells (100–200 cells in each experiment) with aberrant punctate structures along with the nuclear rim was determined in the experiment in (a). Data are shown as the mean ± SEM of three independent experiments. c Electron microscope images of HeLa cells treated with siRNA as described in a and infected with HSV-1 for 22 h. C cytoplasm, N nucleus, NM nuclear membrane. Bars, 500 nm. Images are representative of three independent experiments. d The percent of perinuclear enveloped virions in 15 cells in the experiment in (c) was determined. Data are shown as the mean ± SEM and are representative of three independent experiments. e HeLa cells treated with siRNA as described in (a) were infected with HSV-1 at an MOI of 10 or 0.05 and viral titers were assayed at the indicated times post infection. Data are shown as the mean ± SEM of five (MOI 10) or six independent experiments (MOI 0.05). The indicated P-values were obtained using the Tukey’s test (b, d, e). n.s., not significant