Fig. 8.

ESCRT-III is required for efficient vesicle-mediated nucleocytoplasmic transport in Drosophila S2 cells. a Confocal microscope images of S2 cells treated with control dsRNA (dsRNA-Ct) or dsRNA to shrub (dsRNA-shrub), the Drosophila ortholog of mammalian CHMP4. Arrowheads indicate the Fz2 foci associated with the nucleus. Bars, 5 μm. Images are representative of three independent experiments. b The Fz2-positive punctate structures in dsRNA-treated S2 cells in the experiment in (a) were quantified. Data are shown as the mean ± SEM for 100 cells and are representative of three independent experiments. c Electron microscope images of S2 cells treated with dsRNA-Ct or dsRNA-shrub. Arrowheads indicate the INM invagination structures with large electron-dense granules. C cytoplasm, N nucleus, NM nuclear membrane. Bars, 500 nm. Images are representative of three independent experiments. d The invagination structures with electron-dense granules in dsRNA-treated S2 cells in the experiment in (c) were quantified. Data are shown as the mean ± SEM for 101 cells and are representative of 3 independent experiments. The indicated P-values were obtained using the Tukey’s test (b, d)