Fig. 9.

ESCRT-III contributes to maintenance of the integrity of the inner nuclear membrane in normal HeLa cells. a Confocal microscope images of HeLa, HeLa/CHMP4B KO, and HeLa/ALIX-low cells stained with Lamin A/C (top row) or Emerin (bottom row). Bars, 20 μm. Images are representative of 3 independent experiments. b The number of trans-nuclear tubes in the experiments in (a) was measured (n = 74 for HeLa, n = 55 or HeLa/CHMP4B KO, n = 63 for HeLa/ALIX-low). Data are shown as the mean ± SEM and are representative of three independent experiments. The indicated P-values were obtained using the Tukey’s test. c Electron microscope images of the cells in (a). Arrowheads indicate trans-nuclear tubes derived from the INM. C cytoplasm, N nucleus, NM nuclear membrane. Bars, 500 nm. Images are representative of three independent experiments